Stellaris

Configuration

Manufacturer:
Leica Microsystems
Model: Stellaris 5 (Point Scanning Confocal Microscope)

Applications:
• fixed and unfixed samples (immunofluorescence or fluorescence reporters)
• Microscope live-cell imaging

Body:
• Leica DMi8 CS Premium platform on pressured air table
• Inverted
• LED light source - filter cubes:
- CFP
- GFP
- YFP
- DsRed
• Brightfield light source
• Transmitted light detector
• Motorized xyz scanning stage
- Universal flexible specimen frame

Lasers:
• STELLARIS 5 extended pulsed White light laser: 485nm - 790nm
• diode lasers 405nm and 448nm

Detectors:
5 Power Hybrid HyD S detectors
• Analog, Reflection and Photon Counting detection mode
• Tau Sense capabilities: TauGating, TauContrast, TauSeparation

Objectives:
• 10X HC PL FLUOTAR (NA 0.3; DRY; WD 11 mm)
• 20X HC PL APO (NA 0.75; IMM Water, Gly, Oil; WD 0.62 mm; CS2)
• 25X HC FLOUTAR (NA 0.95; IMM Water, WD 2.5 mm; W)
• 40X HC PL APO (NA 1.1; Water; WD 0.62 mm; CS2)
• 63X HC PL APO (NA 1.2; Water/SALT Water; WD 0.3 mm; CS2)
• 63X HC PL APO (NA 1.3; Gly; W/ 0.3 mm; CS2)
• 100X HC PL APO (NA 1.4; OIL; WD 0.13 mm; CS2)

Scanner, Speed, Resolution:

• 8 kHz Tandem Scanner – switchable between:
o Non-resonant mode:
• confocal point scanner for highest axial resolution (adjustable speed 1-2600 Hz)
• up to 10 fps 512 x 512 px
• up to 131 fps 512 x 16 px
• up to 5200 lines/second
• max resolution 8192 x 8192 px
• zoom 0.75X – 48X
• scan field: 22 mm diagonal

o Resonant mode:
• Line frequency 16000 Hz (bidirectional)
• up to 28 fps at 512 x 512 px
• up to 290 fps at 512 x 16 px
• max resolution 2496 x 2496 px with up to 3 channels simultaneously
• zoom 1.25X – 48X

Software:
• LAS X
• Image Compass: routine multichannel/Z-stack acquisition
• Navigator basic package: tile scan, merge
• LAS X 3D Visualization
• TauSense capabilities: TauGating, TauContrast, TauScan
• Dynamic Signal Enhancement
• LIGHTNING basic detection package
• LAS X Live Data Mode
• LAS X Assay Editor
• LAS X Dye Finder
• LAS X MicroLab (for FRAP and FRET experiments)

Aivia Go
• Semi-automated analysis and editing tools
• 3D objects generated
• Pixel and object colocalization analysis
• Render multi-channel 2 to 4D images of up to 3 TB
• Virtual reality integration for interactively navigating